A Simple Key For thr77 Unveiled
A Simple Key For thr77 Unveiled
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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has been noticed in many tumors, which results in chromosome instability, and therefore may possibly lead to tumorigenesis. This protein was discovered to associate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in cell-cycle regulated histone gene expression and performs a vital function in promoting mobile-cycle progression while in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, have already been explained. Two added splice variants were being described but in depth nucleotide sequence details just isn't still offered.
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Blocking peptides are peptides that bind specially towards the focus on antibody and block antibody binding. These peptide ordinarily includes the epitope identified by the antibody.
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This guidebook outlines even more probable optimization of cellular and immunoassay parameters to be certain the best possible success are...
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This information outlines further doable optimization of mobile and immunoassay parameters to ensure the best possible effects are...
Antibodies bound to the blocking peptide now not bind for the epitope to the target protein. By comparing the staining in the blocked antibody compared to the antibody on your own, one can see which staining is specific.
Goal Information and facts Cyclin E belongs for the hugely conserved cyclin relatives, whose customers are characterized by a extraordinary periodicity in protein abundance from the cell cycle. Cyclins function as regulators of CDK kinases. Distinctive cyclins show distinct expression and degradation styles which add on the temporal coordination of each mitotic occasion. Cyclin E forms a fancy with and functions for a regulatory subunit of CDK2, whose action is required for cell cycle G1/S changeover. This protein accumulates within the G1-S period boundary and is also degraded as cells progress through S section.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both antibodies...
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